Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume
44, Number 9, October 2006, pp.535-537
A Simple, Sensitive,
and Rapid Method for the Determination of Cotinine in Urine by
High-Performance Liquid Chromatography with UV Detection
Lydia Rabbaa-Khabbaz[1], Rita Abi Daoud[1], and
Dolla Karam-Sarkis[2]
[1]Faculty of Pharmacy, Saint-Joseph University, Mathaf, Beirut, Lebanon, P.O.
Box 11-5076 and
[2]Faculty of Pharmacy, Saint-Joseph University and Hôtel-Dieu de France
Hospital, Lebanon
Cotinine levels in biological fluids are
a reliable indicator of the presence of nicotine. In this paper,
a simple and sensitive high-performance liquid chromatography
(HPLC) procedure for the determination of cotinine in urine
following liquid–liquid extraction with dichloromethane
in an alkaline medium is described. Calibration curves show
linearity over the 50 to 3000 ng/mL range with low intra- and
interday variability as well as good selectivity and specificity.
No solid-phase extraction is performed because the liquid dichloromethane
extraction step yields excellent results. This method is a
good alternative for routine analysis of urinary cotinine in
laboratories where gas chromatography or HPLC–mass spectrometry
is not available.
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