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Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 44,
Number 10, November/December 2006, pp.631-633
A Fast, Sensitive HPLC Method for the Determination
of Esterase Activity on a-Tocopheryl Acetate
El Hassan Ajandouz[1], Stéphane Castan[1],
Stefan Jakob[2], and Antoine Puigserver[1]
[1]Institut Méditerranéen de Recherche en Nutrition,
INRA-UMR 1111, Faculté des Sciences et Techniques de Saint-Jérôme,
Université Paul Cézanne-Aix Marseille III and
[2]Adisseo France S.A., 42 avenue Aristide Briand, 92160 Antony,
France
Although data on the behavior of tocopheryl acetate
(Tac) in the gut is rare, some studies show that this ester is
hydrolyzed in the intestine and the released tocopherol (Tol)
is absorbed. An HPLC method is published for the simultaneous
analysis of Tol and Tac in mixed micelles. This study shows how
this method is used for the measurement of esterase activity on
Tac using commercial cholesterol esterase. The rate of hydrolysis
is determined in less than 20 min on the basis of both Tac disappearance
and Tol appearance. The present method is useful for investigating
esterase activities on Tac in model systems that mimick the absorption
environment or in any biological or nonbiological medium.
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