Published:Journal of Chromatographic Science,
ISSN 0021-9665 Volume 44,
Number 10, November/December 2006, pp.591-595
A Uniform HPLC Method Developed for the Analysis
of Salvia miltiorrhiza, Panax notoginseng, and Fufang Danshen
GuiFeng Zeng[1], JianXun Liu[2], LongXing Wang[1],
Qing Xu[1], HongBin Xiao[1], and XinMiao Liang[1],
[1]Dalian Institute of Chemical Physics, Chinese Academy of Sciences,
Dalian 116023, China and
[2]Department of Basic Medical Sciences, XiYuan Hospital, China
Institute of TCM, Beijing 100091, China
Fufang Danshen (FFDS) is a famous typical Chinese
complex prescription, which is mainly composed of Radix Salvia
miltiorrhiza Bunge (SM) and Radix Panax notoginseng (PN). An HPLC
method is developed to analyze SM, PN, and FFDS effectively; the
effective analysis is achieved by using a gradient elution procedure
with a mobile phase consisting of acetonitrile and 0.025% aqueous
phosphoric acid (v/v). Through this method, 33 peaks in FFDS are
clearly exhibited, and the components that make up the 33 peaks
in FFDS are evaluated. Also, the chemical ingredients are compared
between the single herbs (SM and PN) and the complex prescription
(FFDS). The result indicate that the chemical ingredients in FFDS
are not simply a combination of SM and PN. In addition, the HPLC
method is suitable for the routine quality control of SM, PN,
and FFDS, which could present a uniform quality control method
for single medicines and one of the most commonly used Traditional
Chinese Medicine-complex prescriptions.
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