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Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
44, Number 4, April 2006, pp. 227-300
Determination of Geniposide in Rat Urine after Oral Administration
of the Traditional Chinese Medicinal Preparation Yin-Zhi-Ku Decoction by High-Performance
Liquid Chromatography
Determination of Geniposide in Rat Urine after Oral Administration of the Traditional Chinese Medicinal Preparation Yin-Zhi-Ku Decoction by High-Performance Liquid Chromatography
Guan Ye, Hai-Yan Zhu, Hao-Long Zhao, Bo Xu, and Cheng-Gang Huang
Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences,
Chinese Academy of Sciences, Shanghai 201203, P. R. China
A high-performance liquid chromatography method with solid-phase
extraction is introduced for the determination of geniposide in rat urine after
oral administration of Yin-Zhi-Ku decoction. Geniposide and an internal standard
(paeoniflorin) are extracted from urine using Strata cartridges. Analysis of
the extract is then performed on a reversed-phase C18 column using acetonitrile–water
(14:86, v/v) as eluting solvent system. UV detection is set at 238 nm. The calibration
curve for geniposide is linear (r = 0.9996) in the concentration range of 2.0–240
µg/mL. Both intra- and interday precision of the geniposide are determined,
and their relative standard deviation does not exceed 10%. The validated method
is successfully applied to determine geniposide from rat urine after oral administration
of Yin-Zhi-Ku decoction.
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