Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
44, Number 4, April 2006, pp. 205-208
Determination of the Newer Quinolones Levofloxacin and Moxifloxacin
in Plasma by High-Performance Liquid Chromatography with Fluorescence Detection
S. Schulte[1], T. Ackermann[1], N. Bertram[2], T. Sauerbruch[1],
and W.D. Paar[1]
[1]Department of General Internal Medicine, University of Bonn, Sigmund-Freud-Strasse
25, Bonn and [2]LTA-Laboratory for Toxicology and Analytics, Friedrichhöher
Strasse 28, Königswinter, Germany
A simple, accurate, sensitive, and precise reversed-phase (RP)
high-performance liquid chromatographic (HPLC) method with fluorescence detection
allowing the sensitive and specific quantitation of the newer fluoroquinolones
levofloxacin and moxifloxacin is described. Moxifloxacin is used as the internal
standard for the determination of levofloxacin and vice versa. A single-step
liquid–liquid extraction from human plasma is sufficient for both quinolones.
The method is linear from 0.1 to 15 µg/mL and 0.2 to 7 µg/mL for
levofloxacin and moxifloxacin, respectively, covering the clinically relevant
plasma concentration range. The limits of quantitation are 0.05 µg/mL
(levofloxacin) and 0.2 µg/mL (moxifloxacin). The method is successfully
applied to plasma drug level monitoring in a volunteer receiving single therapeutic
doses of levofloxacin or moxifloxacin at two different occasions.
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