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Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
44, Number 4, April 2006, pp. 193-199
Study on a New Precolumn Derivatization Method in the Determination
of Metformin Hydrochloride
Jiang Juan Juan, Feng Fang, Ma Ming, and Zhang Zheng Xing
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing
210009, China
Metformin hydrochloride is successfully determined by reversed-phase
high-performance liquid chromatography with a new precolumn derivatization method
using 9,10-anthraquinone-2-sulfonyl chloride as the derivatization agent. Several
derivatization systems are tried to optimize the derivatization conditions,
and a new post-derivatization treatment method is established. The derivatization
product is analyzed on a Lichrosper C18 column (6.0 mm ¥ 150 mm, 5 µm)
at 256 nm with methanol–water (70:30, v/v) as the mobile phase. The calibration
curves of the derivatives for the UV detector (0.01–4 mg/L) are linear
with respect to peak area. The detection limit (peak area) for the metformin
hydrochloride is 0.01 mg/L for a signal-to-noise ratio of 3:1. In human plasma,
the detection limit is 0.02 mg/L. This assay is rapid, sensitive, and highly
reproducible.
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