Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
44, Number 4, April 2006, pp. 177-180
Preparative High-Performance Liquid Chromatographic Separation
of Naphthodianthrones from St. John’s Wort
S. Puri, G. Handa, A.K. Kalsotra, V.K. Gupta, A.S. Shawl, O.P.
Suri, and G.N. Qazi,
Regional Research Laboratory, Canal Road, Jammu-180001, India
St. John’s Wort (Hypericum perforatum), a perennial flowering
plant, has been used medicinally for thousands of years and has most recently
been identified as an effective treatment for mild to moderate depression and
neuralgic disorders. This work presents a procedure for the isolation of naphthodianthrones
from St. John’s Wort by an accelerated extraction and separation of marker
compounds by preparative high-performance liquid chromatography (HPLC) with
photodiode array detection. The accelerated extraction method minimizes the
extraction time and increases the yield, and the marker compounds obtained by
preparative HPLC are of 98% purity. The compounds are characterized by liquid
chromatography–mass spectrometry (electrospray ionization) and NMR spectra.
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