Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 8, September 2003, pp. 444-446
A Simple and Reliable Semipreparative High-Performance Liquid
Chromatography Technique for the Isolation of Marker-Grade Hyperforin from
Hypericum perforatum L. Extract
R. Anand, S.C. Puri, N. Verma, G. Handa, R.K. Khajuria, V.K.
Gupta, O.P. Suri, and G.N. Qazi
Regional Research Laboratory, Canal Road, Jammu-Tawi (J&K), India –180001
The present work describes isolation of bioactive lipophilic constituent
[namely, hyperforin from St. John’s wort (Hypericum perforatum L.)],
of approximately 98% purity by semipreparative high-performance liquid chromatography
(LC). The extraction, isolation, and analysis of the collected compound is
performed without the use of antioxidants and inert gas atmospheres at all
the stages. Hyperforin, separated isocratically on a 12-µm semiprep column,
is obtained in high purity, lyophilized after the removal of the organic phase,
and preserved at a low temperature. The purity of the collected marker compound
is estimated by the use of LC–mass spectrometry and spectroscopic techniques.
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