Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 8, September 2003, pp. 434-438
Simultaneous Analyses of Neutral Carbohydrates and Amino Sugars
in Freshwaters with HPLC–PAD
Xianhao Cheng[1],[2], and Louis A. Kaplan[1]
[1]Stroud Water Research Center, 970 Spencer Road, Avondale, PA 19311 and
[2]Present
address: Philadelphia Water Department, 1500 E. Hunting Park Avenue, Philadelphia,
PA 19124
In this study, we determine concentrations of neutral and amino
sugars and a sugar alcohol in freshwaters using high-performance liquid chromatography
and pulsed amperometric detection with a single isocratic analysis. Coeluting
arabinose, galactosamine, and mannosamine are separated with a mobile phase
of 22.8mM NaOH-KOH at a temperature of 17°C. The resolutions are 0.73 and
0.64, respectively. The method separates closely eluting glucose–mannose
and mannose–xylose peaks with resolutions of 0.85 and 0.71. Other sugars,
fucose, rhamnose, galactose, fructose, ribose, glucosamine, and mannitol are
resolved completely. Arabinose and galactosamine are measured in stream, ground,
and soil waters that contain dissolved total saccharide (DTS) concentrations
of 527 to 1555nM. Failure to distinguish galactosamine from arabinose in those
samples results in a 53–82% overestimation of arabinose concentrations
and a 1.8–6.5% overestimation of DTS concentrations. The near unity of
glucosamine and galactosamine concentrations in stream water samples allows
us to suggest a correction factor for historical samples that had been analyzed
without resolving galactosamine and arabinose.
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