Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 8, September 2003, pp. 428-433
Characterization of Phthalides in Ligusticum chuanxiong by Liquid
Chromatographic–Atmospheric Pressure Chemical Ionization–Mass Spectrometry
Xiaozhe Zhang, Hongbin Xiao, Qing Xu, Xiuling Li, Jianing Wang,
and Xinmiao Liang*
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 161 Zhongshan
Road, Dalian 116011, P.R. China
High-performance liquid chromatography (HPLC) with diode-array
detection interfaced to atmospheric pressure chemical ionization (APCI)–mass
spectrometry (MS) is applied to analyze phthalides from Chuanxiong (the rhizome
of Ligusticum chuanxiong). This herb material, containing plenty of phthalide
compositions, is selected as the analytical target in this paper for its hematological
activity. Some of the phthalides are not stable and are difficult to analyze
by gas chromatography–MS. Under optimized LC–MS–MS conditions,
six phthalides in the methanol extract of Chuanxiong are unambiguously identified,
and characteristic fragments are obtained using homemade reference standards.
Ten other phthalides in the extract are confirmed by means of LC–APCI–MS
with positive–negative ion mode and collision-induced dissociation in
combination with UV spectrophotometry. The results show that LC–MS–MS
is a method of choice for fast detection and detailed structural analysis of
such mixtures in the crude extract of Chuanxiong.
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