Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 8, September 2003, pp. 418-421
Development and Validation of a Liquid Chromatographic Method
for Monitoring of Process-Related Synthetic Organic Impurities of Profenofos
in Technical Products
R. Nageswara Rao[1], D. Naga Raju[1], N. Venkateswarlu[1],
B. Vittal Rao[2], N. Parvathi[2], A. Manjula[2], G.N. Reddy[2], P.B. Gawali[2],
M. Sreekanth[3], and P. Nageswara Rao[3]
[1]HPLC/UV Group, Analytical Chemistry Division and
[2]Organic Chemistry-II
Division, Indian Institute of Chemical Technology, Uppal Road, Hyderabad-500
007, India
and
[3]Deparment of Chemistry, National Institute of Technology, Warangal-506009,
India
A simple and rapid reversed-phase
high-performance liquid chromatographic method for the monitoring of process-related
synthetic organic impurities of profenofos (PFS) is developed. Impurities are separated
and determined on a reversed-phase Hypersil C18 column using gradient elution
of 50mM ammonium formate buffer–acetonitrile as a mobile phase and detection
at 230 nm at ambient temperature. The method is validated with respect to accuracy,
precision, linearity, and limits of detection and quantitation. The method is
found to be suitable not only for monitoring the reactions involved in the process
development of PFS, but also quality assurance, as it can detect impurities at
the level of 1.5 x 10–8 g.
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