Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 8, September 2003, pp. 393-397
A Rapid High-Performance Liquid Chromatographic Method for the
Simultaneous Quantitation of Aspirin, Salicylic Acid, and Caffeine in Effervescent
Tablets
MaryJean Sawyer and Vimal Kumar
Bayer HealthCare, Consumer Care Division, 36 Columbia Road, Morristown, NJ
07962
A rapid reversed-phase high-performance
liquid chromatographic procedure is developed and validated for the simultaneous
quantitation
of aspirin, salicylic acid, and caffeine extracted from an effervescent tablet.
The method uses a Hypersil C18 column (5 µm, 15 cm ¥ 4.6 mm) for an
isocratic elution in a water–methanol–acetic acid mobile phase at
a wavelength of 275 nm. The tablets’ buffering effects and acid neutralizing
capacity require an extraction solvent of methanol–formic acid. The range
of linearity for aspirin is 0.5–1.25 mg/mL, caffeine 0.065–0.195
mg/mL, and salicylic acid 0.4–6.0% of aspirin. The overall recovery is
100.2%, 100.7%, and 99.2% for aspirin, caffeine, and salicylic acid, respectively.
Under the conditions of the method, aspirin, caffeine, and salicylic acid are
adequately resolved with proper peak symmetry in less than 7 min.
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