Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 9, October 2003, pp. 494-499
A High-Performance Liquid Chromatographic–Tandem Mass
Spectrometric Method for the Determination of Cethromycin (ABT-773) in Human
Plasma, Bronchoalveolar Lavage Fluid, and Alveolar Cells
Qiulei Ren1, John E. Conte, Jr.2,*, Elisabeth Zurlinden2, and
Emil T. Lin1
1Department of Biopharmaceutical Sciences and 2Department of Epidemiology and
Biostatistics, Infectious Diseases Research Laboratory, University of California,
San Francisco, San Francisco, CA 94117
A method is developed for the specific and sensitive determination
of cethromycin concentrations in plasma, bronchoalveolar lavage (BAL), and
alveolar cells (AC), using a high-performance liquid chromatographic–tandem
mass spectrometry (MS) method. The mobile phase consists of 50% acetonitrile–0.05%
acetic acid–5mM ammonium acetate; the column used is a C8 reversed-phase
stationary phase. The preparation of samples requires a solvent extraction
step. The retention times for cethromycin and the internal standard are approximately
2.0 and 2.7 min, respectively, with a total run time of 3.5 min. Detection
is carried out using electrospray MS in a multiple reaction monitor mode. The
detection limits for cethromycin are 1 ng/mL for plasma and 0.2 ng/mL for BAL
supernatants and AC suspensions. The assay has excellent performance characteristics
and has been used to support a study of the intrapulmonary pharmacokinetics
of cethromycin in human subjects.
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