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Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 9, October 2003, pp. 475-479
A Novel Stationary Phase: Capillary-Channeled Polymer (C-CP)
Fibers for HPLC Separations of Proteins
Dwella K. Nelson and R. Kenneth Marcus*
Department of Chemistry, Howard L. Hunter Laboratories, Clemson University,
Clemson, SC 29635-0973
A novel stationary phase is demonstrated for the separation
of proteins. Capillary-channeled polymer (C-CP) fibers provide a stationary
phase that is characterized by a high surface activity (yielding strong wicking
action) and drastically reduced back pressures. Columns prepared by pulling
approximately 1200 50-µm diameter polypropylene C-CP fibers through stainless
steel tubing with column dimensions of 4.6-mm i.d. and 306-mm length exhibit
reversed-phase characteristics in the separation of the proteins. A gradient
method [95:5 water–acetonitrile (ACN)/propanol (1:1) to 35:65 water–ACN/propanol]
with trifluoroacetic acid added as an ion-pairing agent yields high-quality
separations of superoxide dismutase, hemoglobin, hemocyanin, and myoglobin.
It is believed that the C-CP fiber stationary phase holds a number of promising
traits for applications in both analytical and prep-scale separations of diverse
organic species, including a wide range of biomolecules.
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