Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 9, October 2003, pp. 449-459
Gel and Polymer-Solution Mediated Separation of Biopolymers
by Capillary Electrophoresis
András Guttman*
Torrey Mesa Research Institute, 3115 Merryfield Row, La Jolla, CA 92121
In the age of genomics and proteomics, high-resolution separation
techniques are routinely utilized in an integrated and automated fashion to
solve formidable separation problems and provide the means for large-scale
analysis of biological samples with excellent resolution. By automating the
current manual procedures, capillary gel (CGE) and polymer-solution mediated
electrophoresis greatly enhance the productivity of biopolymer analysis while
also reducing both analysis time and the human intervention necessary from
sample loading to data processing. The advent of this novel and high-performance
bioseparation technique has made it possible to sequence the human genome and
revealed global changes in the genome and proteome level, bringing about a
revolutionary transition in our views of living systems on the molecular basis.
CGE and polymer-solution mediated electrophoresis and related microseparation
methods (e.g., electrophoresis microchips) are quickly becoming important separation
and characterization tools in analytical biochemistry and molecular biology.
This review gives an overview of the key application areas of DNA, protein,
and complex carbohydrate analysis, and summarizes the latest developments on
CGE column technology, including capillary coatings and sieving polymer matrices.
Micropreparative aspects and related microseparation techniques are also discussed.
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