Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 5, May/June 2003, pp 267-270
Molecular Species Analysis of Phosphatidylcholine by Reversed-Phase
Ion-Pair High-Performance Liquid Chromatography
Xueqing Lu[1], Qin Liu[2], Zhihua Wang[3], and Xiaokun Hong[3]
[1]Antigen Express, Inc., 100 Barber Avenue, Worcester, MA 01606;
[2]Division
of Biostatistics and Epidemiology, UMASS Cancer Center, 55 Lake Avenue North,
Worcester, MA 01655; and
[3]Shanghai University of Traditional Chinese Medicine
and Pharmacology, Shanghai 200032, China
The retention behavior of molecular species of phosphatidylcholine
(PC) is studied by reversed-phase (RP) ion-pair high-performance liquid chromatography
(HPLC). Mobile phases contain tetraalkyl ammonium phosphates (TAAPs) in methanol–acetonitrile–water.
The stationary phase is alkyl-bonded silica. Competitive interactions of TAAPs,
analyte solutes, and an RP-HPLC column result in reduced retention of PC molecular
species. PC molecular species are eluted at longer retention times with a larger
size of TAAP in the mobile phase, and an increase in the TAAP concentration
invariably causes a decrease in PC molecular species retention times. There
is a linear correlation between the logarithmic retention factors (k) of PC
molecular species and the total number of carbon atoms of TAAP, and the logarithm
of k values of PC molecular species can be approximated as a linear function
of the logarithm of the counter-ion concentration. There is found to be no
distinct dependence between k values of PC molecular species and the mobile
phase pH.
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