Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 5, May/June 2003, pp 241-244
Simultaneous Determination of Seven Compounds in Snow Lotus
Herb Using High-Performance Liquid Chromatography
Xuehui Zhang*, Minqian Yu, and Jianmin Chen
Institute of Medicinal Plant Development, Chinese Academy of Medical Science
and Peking Union Medical College, Xibeiwang, Haidian district, Beijing 100094,
China
A reversed-phase high-performance liquid chromatographic method
is developed for the quantitative determination of seven components of Snow
Lotus Herb (Saussurea tridactyla Sch.-Bip. ex Hook.f.): umbelliferonglucoside,
luteolin-7-O-b-d-glucoside, rutin, apigenin-7-O-b-d-glucoside, kaempferol-3-O-b-d-glucoside,
apigenin-7-O-b-d-rutinoside, and luteolin. Samples are analyzed by means of
a reverse-phase column (Diamonsil C18) using methanol and water under gradient
conditions as the mobile phase for 60 min. This method offers selectivity,
accuracy, precision, linearity, and ruggedness, as well as efficiency and ease.
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