Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 10, October 2001, pp. 142-144
Separation and Determination of Phospholipids in Biological
Samples by High-Performance Liquid Chromatography
Fengjun Wang*, Yun Zhao, and Pei Wang
Institute of Burn Research, Southwest Hospital, Third Military Medical University,
Chongqing, 400038, China
An isocratic high-performance
liquid chromatographic method is developed for the determination of phospholipids
in biological samples
using a µPorasil silica column and a mobile phase of acetonitrile–methanol–85%
phosphoric acid (90:3:1, v/v/v) at a flow rate of 0.80 mL/min. The effluent is
monitored by a UV detector at 203 nm. With the method reported in this paper,
phosphatidylinostol, phosphatidylserine, phosphatidylethnolamine, and phosphatidylcholine
in biological samples are separated and detected successfully. The method is
simple, rapid, and has excellent precision.
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