Comparison of Different Extraction and Detection Methods for Sugars Using Amino-Bonded Phase HPLC

Published: Journal of Chromatographic Science, ISSN 0021-9665 Volume 41, Number 6, July 2003, pp. 331-333

Mustafa Karkacier, Mustafa Erbas, Mustafa K. Uslu, and Mehmet Aksu
Akdeniz University Faculty of Agriculture, Department of Food Engineering, 07070 Antalya, Turkey

In this study, different methods are compared in order to quantitate individual sugars extracted from apple samples using methanol and water. Glucose, fructose, and sucrose are separated in 20 min using an amino-bonded carbohydrate column and a 75:25 acetonitrile–water mobile phase, followed by UV (190 nm) and refractive index detection. Variations in the sugar profiles are observed using different extraction or detection methods (or both) at a 1.4-mL/min flow rate. The data obtained show differences (p < 0.01) from both extraction or detection methods. The highest contents of free sugars studied occur in samples extracted with water.

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