Influence of Mobile Phase Composition on Retention Factors in Different HPLC Systems with Chemically Bonded Stationary Phases

Published: Journal of Chromatographic Science, ISSN 0021-9665 Volume 41, Number 6, July 2003, pp. 289-294

Wojciech Zapala
Department of Chemical Engineering and Process Control, Chemical Faculty, Rzeszów University of Technology, Al. Powstan´ców Warszawy 6, 35-959 Rzeszów, Poland

The influence of mobile phase composition on the retention of selected test analytes in different normal- and reversed-phase chromatographic systems is studied. A novel adsorption model for an accurate prediction of the analyte retention in the column chromatography with binary mobile phase is proposed. Performance of the model is compared with the retention model reported in the literature. Both models are verified for different HPLC systems by use of three criteria: (a) the sum of squared differences between the experimental and theoretical data, (b) approximation of the standard deviation, and (c) the Fisher test.

Reproduction of editorial content of this journal is prohibited without publisher’s permission.

This article is available in its entirety by fax for $4.00 per page.
Visa or MasterCard accepted.

To order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.

Please indicate JCS volume and issue along with page numbers.