Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 1, January 2003, pp. 26-30
Stereodifferentiation of Chiral Compounds Using Reversed-Phase
Liquid Chromatography Coupled with Capillary Gas Chromatography
María Luisa Ruiz del Castillo*, Esther Gómez
Caballero, and Marta Herraiz
Instituto de Fermentaciones Industriales, CSIC, Juan de la Cierva 3, 28006
Madrid, Spain
A method is described for the enantiomeric quantitation of some
chiral compounds via online coupling of reversed-phase liquid chromatography–gas
chromatography. The evaluation of some variables affecting the experimentation
(i.e., the packing material used in the interface, volume of the transferred
fraction, desorption time, initial temperature of the interface, and purge
time) makes it possible to optimize the recoveries obtained for some chiral
terpenes and lactones using a capillary column of b-cyclodextrin dissolved
in OV-1701. The proposed method allows the enantiomeric analysis of aqueous
matrices obtaining relative standard deviations lower than 9% and detection
limits ranging from 0.26–0.93 ppm for the investigated compounds.
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