Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 1, January 2003, pp. 22-25
Separation of Aliphatic and Aromatic Acids, Aromatic Sulfonates,
Quaternary Ammonium Compounds, and Chelating Agents on a Reversed-Phase Column
Without Ion Pairing
Norman E. Skelly
Impact Analytical, 1910 West Saint Andrews Road, Midland, MI 48640-2696
Cleaning products contain a wide variety of components. These
include inorganic materials, weak acids, anionic surfactants, cationic surfactants,
amphoteric compounds, and nonionic materials. The separation of these generally
requires the use of multichromatographic modes. A system is developed that
would give the maximum information for a cleaning product in a single chromatographic
run. With the use of a hydrophobic, high-carbon-loading, and a relatively hydrophobic
surface reversed-phase packing, such compounds as aromatic sulfonates, quaternary
ammonium compounds, weak acids, nitrilotriacetic acid, ethylenediaminetetraacetic
acid, and nonionic materials may be separated in a single run. The mode of
separation is considered to be a combination of reversed-phase, ion-suppression
reversed-phase, and adsorption chromatography. The separation is made on a
YMC-Packl ODS-AQl column (4.6 x 250 mm, 120 Å). The separation employs
a gradient run starting with 0.01N H2SO4 for 10 min followed by a gradient
for 15 min to 100% acetonitrile and continuing for an additional 5 min with
100% acetonitrile. The flow rate is 1 mL/min, and the separation is monitored
at 210 nm.
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