Published:Journal of Chromatographic Science, ISSN 0021-9665Volume 41, Number 1, January 2003, pp. 17-21

Development and Validation of a High-Performance Liquid Chromatography–Electrospray Ionization– Mass Spectrometry Assay for the Determination of Zaleplon in Human Plasma

Fang Feng, Juanjuan Jiang, Hui Dai, and Jie Wu
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing China , 210009

In this paper, a sensitive and rapid chromatographic procedure using a selective analytical detection method (electrospray ionization–mass spectrometry in selected-ion monitoring mode) in combination with a simple and efficient sample preparation step is first presented for the determination of zaleplon in human plasma. The separation of the analyte, internal standard, and possible endogenous compounds are accomplished on a phenomenex Luna 5-µm C8(2) column (250- ¥ 4.6-mm i.d.) with methanol–water (75:25, v/v) as the mobile phase. In order to optimize the mass detection of zaleplon, several parameters such as ionization mode, fragmentor voltage, m/z ratios of ions monitored, type of organic modifier, and eluent additive in the mobile phase are discussed. An internal standard is selected to guarantee the quantitative accuracy. Each analysis takes less than 6 min. The calibration curve of zaleplon in the range of 0.1–60.0 ng/mL in plasma is linear with a correlation coefficient of > 0.9992, and the detection limit (s/n = 3) is 0.1 ng/mL. The within- and between-day variations (relative standard deviation) in the zaleplon plasma analysis are less than 2.4% (n = 15) and 4.7% (n = 15), respectively. The application of this method is demonstrated for the analysis of zeleplon plasma samples in a Phase-I human pharmacokinetic study.

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