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Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 2, February 2003, pp. 96-99
The Optimization of HPLC–UV Conditions for Use with FTIR
Detection in the Analysis of B Vitamins
Ying Li and Phyllis R. Brown
Department of Chemistry, University of Rhode Island, Kingston, RI, 02881
The water-soluble vitamins thiamine (B1), riboflavin (B2), pantothenic
acid (B5), and pyridoxine (B6) are separated by high-performance liquid chromatography.
The mobile phase, column temperature, and flow rate are optimized so that the
chromatograph can be used with a Fourier-transform infrared (FTIR) detector.
Reproducibility, linearity, and detection limits are evaluated for method validation.
Finally, this method is successfully transferred to liquid chromatography–FTIR
with a standard mixture.
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