Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 2, February 2003, pp. 87-91
Determination of Flavones in Crataegus pinnatifida by Capillary
Zone Electrophoresis
Wenmin Liu[1],[2], Guanhua Chen[1], and Tong Cui[2]
[1]Life Science College, Agriculture University of Hebei, Baoding, 071001,
China and
[2]Food Science and Technology College, Agriculture University
of Hebei,
Baoding, 071001, China
A capillary electrophoretic method for the separation of four
flavones in Crataegus pinnatifida is developed. The four flavones in Crataegus
pinnatifida are separated on baseline within 15 min using 50mM borax buffer
containing 15% acetonitrile and adjusted to pH 8.15 with phosphoric acid. The
detection limits of vitexin-2"-rhamnoside, hyperside, rutin, and vitexin
are 0.35, 0.30, 0.40 and, 0.29 µg/mL, respectively. The recovery of these
flavones is as follows: vitexn-2"-rhamnoside 96.8%, hyperside 99.9%, rutin
97.1%, and vitexin 97.8%. The results are in accordance with those obtained
in the high-performance liquid chromatography system. The content of flavones
is higher in Crataegus pinnatifida leaves than in its fruits, and hyperside
is not detected in either Crataegus pinnatifida fruits or flowers.
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