Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 2, February 2003, pp. 67-72
HPLC–Electrospray Ionization–MS–MS Analysis
of Cephalotaxus harringtonia Leaves and Enhancement of the Extraction Efficiency
of Alkaloids Therein by SFE
Young Hae Choi*
GreenTek21 Co., Ltd., #10-132, Yeomri-dong, Mapo-gu, Seoul 121-870, Korea
Ki-Pung Yoo
Department of Chemical Engineering, Sogang University, Seoul 121-742, Korea
Jinwoong Kim
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul
National University, Seoul 151-742, Korea
A high-performance liquid chromatographic method is developed
for use in the electrospray mass spectrometric (MS)–MS analysis of alkaloids
contained in Cephalotaxus harringtonia leaves. Nine alkaloids having ester
groups can be separated and detected with good sensitivity. The MS and MS–MS
spectra obtained provides information on their chemical structures. Supercritical
fluid extraction is also applied in order to improve the extraction efficiency
of Cephalotaxus alkaloids such as cephalotaxine, harringtonine, homoharringtonine,
and isoharringtonine. When carbon dioxide–methanol–water (80:18:2,
v/v) is used the extraction yield is found to be higher than that using the
other supercritical solvents evaluated and conventional organic solvent extraction.
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