Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
41, Number 2, February 2003, pp. 57-52
Rapid Analysis of Phentolamine by High-Performance Liquid Chromatography
Gregory K. Webster*, Robert R. Lemmer, and Steven Greenwald
Summit Analytical Services, 1467 Summerfield Lane, Howell, MI 48843
A rapid liquid chromatographic method is validated for the quantitative
analysis of phentolamine. Phentolamine exists in three forms for this investigation:
as a mesylate salt, hydrochloride salt, and free base. In solution, phentolamine
dissociates from its salt and is chromatographed as free phentolamine. This
validation confirms the analysis of each form, which is simply based upon molar
mass differences encountered in weighing. As such, both the United States Pharmacopeia
hydrochloride and mesylate standards are used throughout this validation to
demonstrate this equivalency. The validation demonstrates that this method
may be used to quantitate phentolamine, regardless of its salt form.
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