Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume 41, Number 7, August 2003, pp. 377-380
A Clean and Rapid Liquid Chromatographic Technique for Sulfamethazine
Monitoring in Pork Tissues without Using Organic Solvents
Naoto Furusawa
Graduate School of Human Life Science, Osaka City University, Osaka 558-8585,
Japan
A rapid method for the isolation and high-performance liquid
chromatographic (HPLC) determination of sulfamethazine (SMZ) in pork tissues
(kidney, liver, and muscle) without using organic solvents is developed. The
isolation is performed by homogenization with an acid solution using an ultrasonic-homogenizer,
followed by centrifugation. The HPLC analyses are performed using a reversed-phase
C4 column (150- ¥ 4.6-mm i.d.), a mobile phase of 0.02 mol/L citric acid
solution, and a photodiode array detector. The resulting HPLC chromatograms
are free from interferences for determination and identification. The proposed
technique is shown to be linear (r > 0.99) over the concentration range
0.1–2.0 µg/g for all pork tissues. Average recoveries of SMZ (spiked
0.1–2.0 µg/g) range from 87.6% to 90.2%, with inter- and intra-assay
variabilities of less than 4%. The total time required for the analysis of
one sample and limit of quantitation is less than 20 min and 0.09 µg/g,
respectively.
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