Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume 41, Number 7, August 2003, pp. 337-342
Evaluation of a New Reagent: Anthraquinone-2-Sulfonyl Chloride
for the Determination of Phenol in Water by Liquid Chromatography Using Precolumn
Phase-Transfer Catalyzed Derivatization
Fang Feng[1], Bunji Uno[2], Massashi Goto[2], Zhengxi Zhang[1],
and Dengkui An[1]
[1]Department of Pharmaceutical Analysis, China Pharmaceutical University,
Nanjing 210009, China and
[2]Department of Pharmaceutical Analysis, Gifu Pharmaceutical
University, Gifu 502-8585, Japan
A new reagent, anthraquinone-2-sulfonyl chloride, is used for
the derivatizaton of phenols. Several compounds with different polarities are
selected to evaluate the new reagent and derivatives of these phenols that
are prepared via a facile pathway. The optimal conditions for analytical derivatization
and mechanism of the derivatization reaction are discussed. The derivatization
procedure involves an ion-pair extraction of the deprotonated phenols with
a tetrabutylammonium counter ion in the organic phase. At the interface of
two phases, the derivatization reaction occurs quantitatively at room temperature
within 3 min. The derivatives are stable and readily amenable to analysis by
normal-phase (NP) and reversed-phase (RP) high-performance liquid chromatography
(HPLC). Excellent linearity response was demonstrated over the concentration
range of 0.2–200 µmol/L at 320 nm for NP-HPLC and at 256 nm for
RP-HPLC. Combined with preconcentration using a Waters Sep-Pak Plus C18 cartridge,
detection limits of phenols for water-sample analysis are as low as 1 ¥ 10–9
mol/L (~ 0.1 µg/mL).
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