Published:Journal of Chromatographic Science, ISSN 0021-9665Volume 41, Number 7, August 2003, pp. 337-342

Evaluation of a New Reagent: Anthraquinone-2-Sulfonyl Chloride for the Determination of Phenol in Water by Liquid Chromatography Using Precolumn Phase-Transfer Catalyzed Derivatization

Fang Feng[1], Bunji Uno[2], Massashi Goto[2], Zhengxi Zhang[1], and Dengkui An[1]
[1]Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China and
[2]Department of Pharmaceutical Analysis, Gifu Pharmaceutical University, Gifu 502-8585, Japan

A new reagent, anthraquinone-2-sulfonyl chloride, is used for the derivatizaton of phenols. Several compounds with different polarities are selected to evaluate the new reagent and derivatives of these phenols that are prepared via a facile pathway. The optimal conditions for analytical derivatization and mechanism of the derivatization reaction are discussed. The derivatization procedure involves an ion-pair extraction of the deprotonated phenols with a tetrabutylammonium counter ion in the organic phase. At the interface of two phases, the derivatization reaction occurs quantitatively at room temperature within 3 min. The derivatives are stable and readily amenable to analysis by normal-phase (NP) and reversed-phase (RP) high-performance liquid chromatography (HPLC). Excellent linearity response was demonstrated over the concentration range of 0.2–200 µmol/L at 320 nm for NP-HPLC and at 256 nm for RP-HPLC. Combined with preconcentration using a Waters Sep-Pak Plus C18 cartridge, detection limits of phenols for water-sample analysis are as low as 1 ¥ 10–9 mol/L (~ 0.1 µg/mL).

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