Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 10, October 2001, pp. 209-214
Fast HPLC with a Silica-Based Monolithic ODS Column
José M. Santiuste
Instituto de Química Física “Rocasolano”, CSIC, Department
of Structure and Molecular Dynamics, C. Serrano, 119, 28006-Madrid, Spain
Fast high-performance liquid chromatography is becoming routine
in laboratories that require high throughput or for combinatorial libraries.
Reduced analysis time is commonly achieved by using shorter columns and higher
flow rates. Shorter columns require smaller particles in order to maintain
efficiency. However, smaller particles increase backpressure, which limits
both column length and higher flow rates for typical LC pumps. This disadvantage
has been addressed by the emergence of monolithic liquid chromatographic columns
(1). Unlike particle-base columns, monolithic columns consist of a continuous
rod-shaped porous network with a bimodal pore distribution. In this study,
a commercially available 50- ¥ 4.6-mm silica-based octadecyl silane monolithic
column (Chromolith SpeedROD RP18e, EM Science, Gibbstown, NJ) was used to separate
a seven-component test mixture with a wide range of polarity. The primary goals
of this investigation were: (a) to study the chemistry (selectivity) of the
new silica-based monolithic columns and (b) to study their run-to-run and column-to-column
performance (retention times and peak areas). The selectivity (a factor) is
a ratio of partition coefficients and, if comparable for a variety of solutes,
would mean that methods could be readily transferred between particulate and
monolithic columns.
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