Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 10, October 2001, pp. 195-199
Simultaneous Determination of Four Heavy Metal Ions in Tobacco
and Tobacco Additive by Online Enrichment Followed by RP-HPLC and Microwave
Digestion
Guangyu Yang[1],[2], Chengming Zhang[2], Qiufen Hu[1], and
Jiayuan Yin[1]
[1]Department of Chemistry, Yunnan University, Kunming 650091, P.R. China and
[2]Key Laboratory of Chemistry & Engineering, Yunnan Academy of Tobacco
Science,
Kunming 650106, P.R. China
A new method for the simultaneous determination of tin, lead,
cadmium, and mercury in tobacco and tobacco additive by reversed-phase high-performance
liquid chromatography combined with microwave digestion and an online enrichment
technique is developed. The tin, lead, cadmium, and mercury ions are precolumn
derivatized with tetra-(4-dimethylaminophenyl)-porphyrin (T4-DMAPP) to form
color chelates. The Sn-T4-DMAPP, Hg-T4-DMAPP, Cd-T4-DMAPP, and Pb-T4-DMAPP
chelates are absorbed onto the front of the enrichment column using a buffer
solution of 0.05 mol/L pyrrolidine–acetic acid (pH = 10.0) as the mobile
phase. After the concentration is finished (by switching the six-port switching
valve) the retained chelates are back-flushed by the mobile phase and move
to the analytical column. The chelate separation on the analytical column is
satisfactory using gradient elution with methanol (containing 0.05 mol/L pyrrolidine–acetic
acid buffer salt, pH = 10.0) and tetrahydrofuran (containing 0.05 mol/L pyrrolidine–acetic
acid buffer salt, pH = 10.0). The linearity range is 0.01–120 µg/L
for each metal ion. The detection limits (S/N = 3) of tin, lead, cadmium, and
mercury are 0.6, 0.8, 0.5, and 0.6 ng/L, respectively. This method is applied
to the determination of tin, lead, cadmium, and mercury in tobacco and it’s
additive with good results.
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