Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 10, October 2001, pp. 182-186
Capillary Electrophoretic Behaviors of Pharmacologically Active
Xanthones from Securidaca inappendiculata with b-Cyclodextrin as a Buffer Additive
Tao Bo[1], Yongfa Huang[2], Xuedong Yang[3], Ke An Li1, Huwei
Liu[1], and Lizhen Xu[3]
[1]Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry
of Education, College of Chemistry and Molecular Engineering, Peking University,
Beijing, 100871, P.R. China;
[2]Jiu Zhi Tang Inc., Chang sha, 410008, P.R.
China; and
[3]Institute of Medicinal Plant Development, Peking Union Medical
College
and Chinese Academy of Medical Sciences, Beijing, 100094, P.R. China
The capillary electrophoretic (CE) behaviors of ten xanthones
in the presence of b-cyclodextrin (CD) are investigated, and apparent analyte-selector
binding constants between b-CD and the xanthones in the CE running buffer are
calculated to elucidate the migration order. Also, the separation selectivity
with b-CD additive is compared with that of sulfated b-CD additive. It is indicated
that b-CD can greatly change the separation selectivity of xanthones, and the
electrophoretic behaviors of xanthones are rather different when using b-CD
from that when using sulfated b-CD as an additive.
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