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Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 10, October 2001, pp. 177-181
HPLC Analysis of Metabolically Produced Formaldehyde
J. Lengyel[1], H. Kalász[1], T. Szarvas[2], Cs. Peltz[3],
and A. Szarkáné-Bolehovszky[1]
[1]Semmelweis University, 1089 Budapest, Nagyvárad ter 4, Hungary;
[2]Institute
of Isotopes Co., Ltd., Budapest, Hungary;
[3]Department of Theoretical Chemistry,
Eötvös Loránd University, Budapest, Hungary
Radiolabelled (–)-deprenyl is orally administered to rats,
and urinary elimination of radiolabelled formaldehyde is detected. The separation
is performed using high-performance liquid chromatography on octadecyl-silica
stationary phase. Both the radioactivity and the UV absorbance of the dinitrophenylhydrazine
formaldehyde peak are determined. Formaldehyde generation takes place by N-demethylation.
Low levels of formaldehyde may have a beneficial role in counterbalancing the
oxidative stress of the everyday person’s life.
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