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Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 10, October 2001, pp. 173-176
Determination of Antibacterial Flomoxef in Serum by Capillary
Electrophoresis
Toshihiro Kitahashi* and Itaru Furuta
Department of Laboratory Medicine, Kinki University School of Medicine, 377-2
Ohno-higashi, Osakasayama, Osaka, Japan
A determination method of flomoxef (FMOX) concentration in serum
by capillary electrophoresis is developed. Serum samples are extracted with
acetonitrile. After pretreatment, they are separated in a fused-silica capillary
tube with a 25mM borate buffer (pH 10.0) as a running buffer that contains
50mM sodium dodecyl sulfate. The FMOX and acetaminophen (internal standard)
are detected by UV absorbance at 200 nm. Linearity (0–200 mg/L) is good,
and the minimum limit of detection is 1.0 mg/L (S/N = 3). The relative standard
deviations of intra- and interassay variability are 1.60–4.78% and 2.10–3.31%,
respectively, and the recovery rate is 84–98%. This method can be used
for determination of FMOX concentration in serum.
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