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Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
39, Number 10, October 2001, pp. 169-172
Study of the Interaction of Structurally Similar Bioactive Compounds
by Thin-Layer Chromatography
Orsolya Farkas, Erzsébet Gere-Pászti, and Esther
Forgács*
Institute of Chemistry, Chemical Research Center of the Hungarian Academy of
Sciences, P.O. Box 17, 1525 Budapest, Hungary
The interaction between steroids and cholesterol is studied
by reversed-phase thin-layer chromatography (TLC) using TLC plates impregnated
by cholesterol and methanol–water mixtures as the eluents. The RM values
obtained are in linear correlation with the methanol concentration of the eluent.
The slope obtained from the linear regression analysis, which is characteristic
of the strength of interaction, is determined. Stepwise regression and principal
component analysis are carried out to find a relationship between the physicochemical
parameters of steroid drugs and the strength of the interaction, which is followed
by nonlinear mapping and cluster analysis to visualize the obtained results.
The results show that steric and electronic parameters of the steroid drugs
have a significant effect on the strength of the interaction between these
structurally similar molecules.
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