Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 8, September 2002, pp. 429-433
Simultaneous Determination of Ofloxacin, Tetrahydrozoline Hydrochloride,
and Prednisolone Acetate by High-Performance Liquid Chromatography
Mohammed Shahid Ali, Mohsin Ghori, and Anwar Saeed
Jamjoom Pharmaceuticals, P.O. Box No. 6267, Jeddah—21442, Kingdom of Saudi
Arabia
A simple, specific, and precise high-performance liquid chromatographic
method has been developed for the simultaneous determination of ofloxacin (OFX),
tetrahydrozoline hydrochloride (THC), and prednisolone acetate (PAC) in ophthalmic
suspension using propylparaben (POP) as the internal standard. The mobile phase
consists of 0.05M phosphate buffer–acetonitrile (65:35, v/v), and the
pH is adjusted to 2.7 with orthophosphoric acid. A column containing octadecyl
silane chemically bonded to porous silica particles (Waters Spherisorb‚
5 µm ODS 1, 4.6 ¥ 150 mm) is used as the stationary phase. The detection
is carried out using a variable wavelength UV–vis detector set at 210
nm for OFX and THC and 254 nm for POP (internal standard) and PAC. The solutions
are chromatographed at a constant flow rate of 1.2 mL/min. Retention times for
OFX, THC, POP, and PAC are approximately 2.5, 4.5, 7.8, and 9.5 min, respectively.
The relative retention times are approximately 0.14 min for OFX, 0.35 min for
THC, 1.00 min for POP, and 1.22 min for PAC. The linearity range and percent
recoveries for OFX, THC, and PAC are 24–120, 4–16, and 16–80
µg/mL and 100.48%, 100.34%, and 100.21%, respectively.
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