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Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume 40, Number 9, October 2002, pp. 505-508
Chiral Analysis of Biogenic d,l-Amino Acids Derivatized by N-Fluorenylmethoxycarbonyl-l-alanyl N-Carboxyanhydride Using High-Performance Liquid Chromatography
Jaroslav Zbrozek[1], Martin Pumera[2], and Martin Flegel[3]
[1]Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemigovo námestí 2, CZ-166 10 Prague 6, Czech Republic;
[2]Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003; and
[3]PolyPeptide Laboratories, Radiová 1, CZ-10 000 Prague 10, Czech Republic
Nineteen biogenic d,l-amino acids are derivatized with highly reactive N-fluorenylmethoxycarbonyl-l-alanyl N-carboxyanhydride. Using a 0.5M borate buffer at pH 7.5 and acetone, the derivatization of amino acids is completed in 5 min at room temperature. Some of the resulting diastereomeric N-protected dipeptides are successfully separated on an octylsilica stationary phase using 100mM acetate buffer (pH 4.4) and acetonitrile as the eluent.
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