|
Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume 40, Number 9, October 2002, pp. 495-499
Determination of the Norepinephrine Level by High-Performance
Liquid Chromatography to Assess the Protective Effect of MAO-B Inhibitors Against
DSP-4 Toxicity
Diana Haberle, Kálmán Magyar, and Éva
Szökő
Department of Pharmacodynamics, Semmelweis University, Nagyvárad tér
4, Budapest, H-1089 Hungary
Liquid chromatography (LC) combined with electrochemical detection
(EC) is suitable for measuring oxidizable biogenic amine levels in small samples
of brain tissue. The norepinephrine (NE) content in mouse hippocampus after
treatment with various monoamine oxidase-B enzyme (MAO-B) inhibitors ([–]-deprenyl,
[+]-rasagiline, and the noradrenergic neurotoxin N-[2-chloroethyl]-N-ethyl-2-bromobenzylamine
[DSP-4]) is determined using an LC–EC method. Treatment with a single
intraperitoneal dose of (–)-deprenyl (selegiline) before DSP-4 administration
markedly reduces the NE depleting effect of the toxin, and (+)-rasagiline does
not significantly modify the NE level decreased by the neurotoxin. The MAO-B
inhibitory potency of (–)-deprenyl and (+)-rasagiline is also evaluated.
Significantly reduced MAO-B enzyme activity in mouse brain and liver is measured
6 h after treatment with their single dose. (+)-Rasagiline is found to be a
more potent MAO-B inhibitor than (–)-deprenyl.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted. To
order electronically
click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers. |
Site Map: Home
| Current Issue | Subscribe
| Back Issues | About
Us | Meetings | Advertising
|
| Books for Sale | For
the Author | Links | Supplier
Info | Search | |
