Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume 40, Number 9, October 2002, pp. 483-487
Selectivity Tuning in Chiral Dual Column Gas Chromatography
J. Krupcík[1], I. Spánik[1], E. Benická1,
M. Zabka[1], T. Welsch[2], and D.W. Armstrong[3]
[1]Department of Analytical Chemistry, Faculty of Chemical Technology, Slovak
Technical University, Radlinského 9, 812 37 Bratislava, Slovakia;
[2]Department of Analytical and Environmental Chemistry, University of Ulm,
Einstein-Allee 11, D-89069 Ulm, Germany; and
[3]Department of Chemistry, Gilman Hall, Iowa State University, Ames, IA 50011-3111
The enantioselective tuning of two columns coupled in series
is investigated in chiral high-resolution gas chromatography. Two columns with
opposite enantioselectivities (Chirasil-L-Val and Chirasil-D-Val) are coupled
in series via a T connector, and the relative retention of enantiomers chromatographed
on the system is changed by varying the individual carrier gas flow rates in
the coupled columns. The flow-rate ratio necessary for the required selectivity
is calculated on the basis of the measured retention factors on the individual
columns. The performance of this method for adjusting selectivity is studied
by the separation of enantiomers of the N-TFA-O-methyl esters of six amino acids.
It is demonstrated that the change of the coupling point carrier gas pressure,
at the constant inlet and outlet pressures, may change the enantioselectivity
of the given column series to such an extent that the enantiomer elution order
may be reversed.
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