Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume 40, Number 9, October 2002, pp. 477-482
An Improved Method for Acetaldehyde Determination in Blood by
High-Performance Liquid Chromatography and Solid-Phase Extraction
Daisuke Kozutsumi*, Masayo Arita, Akihiro Kawashima, Mami Adachi,
and Masao Takami
Pharmaceuticals Development Department, Meiji Dairies Corporation, 540 Naruda,
Odawara, Kanagawa 250-0862, Japan
This study reports on an improved method for acetaldehyde (ACH)
determination in blood by high-performance liquid chromatography (HPLC). In
the case of HPLC analysis, ACH is generally converted to derivatives for ultraviolet
detection (for example 2,4-dinitrophenylhydrazine [DNPH] derivative). Nevertheless,
elevation of the background during protein precipitation, hydrazone synthesis,
or both frequently results in a serious loss of accuracy and precision of the
analysis. The method in this study is developed to minimize the increase in
nonspecific ACH–DNPH with a view to optimize mainly the synthetic condition
of ACH–DNPH. The background is decreased dramatically by gentle deproteination,
optimization of the DNPH amount and reaction pH, and reversed-phase solid extraction
for the elimination of excess DNPH reagent. The standard curves show good linearity
between 0 and 100µM and minimal background is observed, indicating that
the method is useful for monitoring the ACH concentration in blood.
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