Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 10, November/December 2002, pp. 603-608
Metabolite Analysis, Isolation and Purity Assessment Using Various
Liquid Chromatographic Techniques Combined with Radioactivity Detection
Gy. Morovján[1], B. Dalmadi-Kiss[1], I. Klebovich[1],
and E. Mincsovics[2]
[1]EGIS Pharmaceuticals Ltd., P.O. Box 100, H-1475 Budapest, Hungary and
[2]OPLC-NIT Engineering Co. Ltd., Andor str. 60, H-1119 Budapest, Hungary
During the discovery of metabolic routes of a drug candidate,
radioactively labeled substances are administered. This study reports the multidimensional
application of overpressured layer chromatography (OPLC) and high-performance
liquid chromatography (HPLC) coupled with online or off-line nondestructive
radioactivity detection methods in metabolism studies. Among these methods,
digital autoradiography and flow-cell radioactivity detectors (RD) using solid
scintillators are used. In this study, the hyphenation of OPLC with RD is reported.
The application of the OPLC–RD technique is demonstrated on a metabolism
study as well as the multidimensional chromatographic selectivity using normal-phase
OPLC for the separation in the first dimension, followed by reversed-phase HPLC–RD,
which provides additional selectivity to the separation. Information regarding
the identity of radiolabeled metabolites and data obtained from spectroscopic
methods could be advantageously used during structure elucidation.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $40.00.
Visa, MasterCard & Amex accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers.
For permission to reuse material from Journal of Chromatographic Science, please access www.copyright.com or contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750-8400. CCC is a not-for-profit organization that provides licenses and registration for a variety of uses.
