Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 10, November/December 2002, pp. 553-563
Planar Chromatographic Method Development Using the PRISMA Optimization
System and Flow Charts
Sz. Nyiredy
Research Institute for Medicinal Plants, H-2011 Budakalász, P.O. Box
11, Hungary
This study presents a modern planar chromatographic method-development
procedure, based on the “PRISMA” optimization system, in which the
optimum separation is achieved systematically and the structures and properties
of the substances to be separated are not known. The procedure consists of three
stages. In the first of these the basic conditions the stationary phase, vapor
phase, and individual solvents are selected with a TLC procedure (generally
in nonsaturated chromatographic chambers). In the second stage, the optimum
combination of the selected solvents is determined with the PRISMA model. The
third part of the procedure includes the selection of the development mode (circular,
linear, or anticircular); the selection of an appropriate forced-flow chromatographic
technique (over-pressured layer chromatography or rotation planar chromatography)
with high-performance thin-layer chromatographic plates; the transfer of the
optimized mobile phase to the various analytical, planar, or column preparative
liquid chromatographic techniques; and the selection of the operating conditions.
For practical reasons, the optimization process is presented with the help of
flow charts.
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