Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 10, November/December 2002, pp. 550-552
Ultrathin-Layer Chromatography
Heinz E. Hauck and Michael Schulz
Merck KGaA, Darmstadt, Germany
The development of ultrathin-layer silica gel plates with a monolithic
structure opens up a new dimension in thin-layer chromatography (TLC). The very
small layer thickness of approximately 10 µm and the absence of any kind
of binder in combination with the framework of this stationary phase lead to
new and improved properties of these ultrathin-layer chromatographic (UTLC)
silica-gel plates compared with conventional TLC and high-performance TLC (HPTLC)
precoated layers. First of all, the advantages of the UTLC plates are the very
short migration distances and, in combination with this, the short development
times as well as the very low consumption of solvents as the mobile phase in
connection with high sensitivity. The separations of amino acids, pesticides,
pharmaceutically active ingredients, phenols, and plasticizers effectively demonstrate
the possibilities of the new ultrathin-layer silica-gel plates. Furthermore,
a comparison of UTLC, HPTLC, and TLC concerning retention behavior, efficiency,
detection limits, migration times, and solvent consumption is performed effectively
by the separation of caffeine and paracetamol.
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