Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 3, March 2002, pp. 156-161
Separation and Identification of the Phthalic Anhydride Derivatives
of Liqusticum Chuanxiong Hort by GC–MS, TLC, HPLC–DAD, and HPLC–MS
Hong-Xia Li, Ming-Yu Ding, and Jian-Yuan Yu
Department of Chemistry, Tsinghua University, Beijing 100084, China
A simple, sensitive, and rapid method using gas chromatography
(GC)–mass spectrometry (MS) is developed for the simultaneous separation
and identification of the active ingredients of Liqusticum Chuanxiong Hort (Chuanxiong).
Ten phthalic anhydride derivatives (PADs) are identified in Chuanxiong as 3-butylphthalide,
3-butylidenephthalide, 3-butylidene-4-hydroxyphthalide, senkyunolide A, neocnidilide,
Z-ligustilide, E-ligustilide, senkyunolide F, senkyunolide-H, and senkyunolide-I.
The existence of ferulic acid and vanillin in Chuanxiong extract is also demonstrated.
Further identification of these compounds is performed by thin-layer chromatography,
high-performance liquid chromatography (HPLC), and HPLC–MS analysis. This
is the first report of the separation and determination of the PADs in Chuanxiong
by GC–MS.
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