Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 3, March 2002, pp. 140-146
Automation of Solid-Phase Microextraction– Gas Chromatography–Mass
Spectrometry Extraction of Eucalyptus Volatiles
Cláudia A. Zini[1],[2], Heather Lord[1], Eva Christensen[3],
Teotônio F. de Assis[4], Elina B. Caramão[2], and Janusz Pawliszyn[1]
[1]Department of Chemistry, University of Waterloo, Waterloo, ON, N2L 3G1, Canada
[2]Institute of Chemistry, Universidade Federal do Rio Grande do Sul (UFRGS),
Porto Alegre, RS, 91501 970, Brazil
[3]MiljØ Kemi – Danish Environmental Center, Lille Tornbjerg Vej
24a, DK-5220, Odense SØ, Denmark
[4]Riocell S/A, P.O. Box 108, 92500 000 Guaíba-RS, Brazil
Solid-phase microextraction (SPME) coupled with gas chromatography
(GC)–ion-trap mass spectrometry (ITMS) is employed to analyze fragrance
compounds from different species of eucalyptus trees: Eucalyptus dunnii, Eucalyptus
saligna, Eucalyptus grandis, and hybrids of other species. The analyses are
performed using an automated system for preincubation, extraction, injection,
and analysis of samples. The autosampler used is a CombiPAL and has much flexibility
for the development of SPME methods and accommodates a variety of vial sizes.
For automated fragrance analysis the 10- and 20-mL vials are the most appropriate.
The chromatographic separation and identification of the analytes are performed
with a Varian Saturn 4D GC–ITMS using an HP-5MS capillary column. Several
compounds of eucalyptus volatiles are identified, with good reproducibility
for both the peak areas and retention times. Equilibrium extraction provides
maximal sensitivity but requires additional consideration for the effect of
carryover. Preequilibrium extraction allows good sensitivity with minimal carryover.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $40.00.
Visa, MasterCard & Amex accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
