Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 6, July 2002, pp. 355-358
Simplified High-Performance Liquid Chromatographic Determination
of Residual Amprolium in Edible Chicken Tissues
N. Furusawa Graduate School of Human Life Science, Osaka City University,
Osaka 558-8585, Japan
A simplified determining method for the routine monitoring of
residual amprolium in edible chicken tissues (muscle and liver) is developed
using a high-performance liquid chromatographic (HPLC) method with a photodiode-array
detector after sample cleanup by an Ultrafree-MC/PL centrifugal ultrafiltration
unit. For the HPLC determination and identification, a Mightysil RP-4 GP column
and a mobile phase of an ethanol–5mM 1-heptanesulfonic acid sodium salt
solution (35:65, v/v) using an ion-pairing system with a photodiode-array detector
are used. Average recoveries (spiked at 0.3–3.0 µg/g) are > 90%.
The inter- and intravariabilities are 1.9–2.4%. The limits of quantitation
are 0.22 µg/g for muscle and 0.25 µg/g for liver. The total time
and solvent required for the analysis of one sample are < 20 min and <
2 mL of ethanol, respectively. No toxic solvents and regents are used.
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