Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 1, January 2002, pp. 49-53
Analysis of Amlodipine in Human Plasma by Liquid Chromatography–Mass
Spectrometry
Yan
Feng*, Liang Zhang, Zheng Shen, Fengyun Pan, and Zhengxing Zhang
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing
210009, People’s Republic of China
A simple, sensitive, and specific liquid chromatographic method
coupled with electrospray ionization (ESI)–mass spectrometry for the determination
of amlodipine is developed. After extraction by ethyl acetate using nicardipine
as the internal standard, solutes are separated on a C18 column with a mobile
phase of methanol–1% HAc (65:35). Detection is performed on an air pressure
ionization single quadruple mass spectrometer equipped with an ESI interface
and operated in positive-ionization mode. Amlodipine quantitation is realized
by computing the peak-area ratio (amlodipine–nicardipine) of the extracts
analyzed in single ion monitoring mode (m/z 431 and m/z 480 for amlodipine and
nicardipine, respectively) and comparing them with the calibration curve (r
= 0.9991).
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