Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 1, January 2002, pp. 35-39
Simultaneous and Direct Determination of Oxalic Acid, Tartaric
Acid, Malic Acid, Vitamin C, Citric Acid, and Succinic Acid in Fructus mume
by Reversed-Phase High-Performance Liquid Chromatography
Chen
Zhanguo* and Lu Jiuru
Department of Chemistry, School of Chemistry and Material Science, Shaanxi Normal
University, Xi’an, 710062, P.R. China
A method for the simultaneous separation and direct determination
of oxalic acid (OA), tartaric acid (TA), malic acid (MA), vitamin C (VC), citric
acid (CA), and succinic acid (SA) in Fructus mume using reversed-phase high-performance
liquid chromatography with a UV detector in an acidic medium is presented in
this study. In the experiment, the optimization of chromatographic conditions
(i.e., the pH and flow rate of the mobile phase, the absorption wavelength,
and temperature of column) that affect the separation degree and peak shape
of organic acids has been obtained. The linear ranges are found to be 0.05–4.7
µg for OA (r = 0.9999), 0.11–10.5 µg for TA (r = 0.9999), 0.114–11.4
µg for MA (r = 0.9999), 0.033–3.30 µg for VC (r = 0.9999),
0.155–15.5 µg for CA (r = 0.9998), and 0.194–19.4 µg for
SA (r = 0.9996). For OA, TA, MA, VC, CA, and SA, the even recovery (n = 3) of
six effective components are 100.9%, 99.97%, 101.2%, 102.1%, 101.1%, and 100.7%,
respectively, and the largest relative standard deviation (n = 11) for the six
components is less than 1.7%. The detection limits are 0.01 µg for OA,
TA, and VC; 0.05 µg for MA; 0.03 µg for CA; and 0.1 µg for
SA. In a single chromatographic run, OA, TA, MA, VC, CA, and SA can be determined
in less than 7 min. The method can be used for the purpose of routine analysis
and the quality control of a botanic (Fructus mume) containing these effective
components.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $4.00 per
page.
Visa or MasterCard accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers.
