Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 2, February 2002, pp. 92-96
The Use of Nonendcapped C18 Columns in the Cleanup of Clenbuterol and a New Adrenergic Agonist from Bovine Liver by Gas ChromatographyTandem Mass Spectrometry Analysis
Maurizio Fiori, Claudia Cartoni, Beatrice Bocca, and
Gianfranco Brambilla
Istituto Superiore di Sanità—Laboratorio di Medicina Veterinaria, viale Regina
Elena 299, I-00161 Rome, Italy
More specific official methodology is needed to survey the illegal
use of clenbuterol in animal production plus the synthesis of new compounds
that currently elude routine analytical methods. The identification of a new
adrenergic agonist, N1-(2-(4-amino-3,5-dichlorophenyl)-2-hydroxyethyl)-N1-isopropyl-propanamide
(known as compound A) in animal feed has prompted studies to verify if the existing
cleanup procedures developed for clenbuterol are really effective. This study
considers the ion-exchange mechanism on cyanopropyl (CN), sulfonic cation exchange
(SCX), mixed phase (MPH) (C8 + SCX), and nonendcapped C18 (C18NE) solid-phase
extraction (SPE) columns. Results indicate that compound A (by contrast with
clenbuterol) is not efficiently retained on the CN, SCX, and MPH SPE columns
(recovery < 10%). This finding thus leads to the development of a gas chromatography–tandem
mass spectrometry procedure based on C18NE SPE that is able to purify both agonists
from bovine livers spiked at 0.5, 1.0, and 2.0 ppb with a mean recovery of 93%
for clenbuterol and 92% for compound A.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $40.00.
Visa, MasterCard & Amex accepted.
To
order electronicallyclick here or call: 847-647-2900 ext. 1323 or fax request to: 847-647-1155.
Please
indicate JCS volume and issue along with
page numbers.
For permission to reuse material from Journal of Chromatographic Science, please access www.copyright.com or contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750-8400. CCC is a not-for-profit organization that provides licenses and registration for a variety of uses.
