Published:Journal of Chromatographic Science,
ISSN 0021-9665Volume
40, Number 2, February 2002, pp. 87-91
Influence of Hydrolysis, Purification, and Calibration Method on Furosine Determination Using Ion-Pair Reversed-Phase High-Performance Liquid Chromatography
M.A. Serrano, G. Castillo, M.M. Muñoz, and A. Hernández
Departamento de Nutrición y Bromatología, Campus Universitario, Universidad
de Alcalá, 28871-Alcalá de Henares (Madrid), Spain
More specific official methodology is needed to survey the illegal
use of clenbuterol in animal production plus the synthesis of new compounds
that currently elude routine analytical methods. The identification of a new
adrenergic agonist, N1-(2-(4-amino-3,5-dichlorophenyl)-2-hydroxyethyl)-N1-isopropyl-propanamide
(known as compound A) in animal feed has prompted studies to verify if the existing
cleanup procedures developed for clenbuterol are really effective. This study
considers the ion-exchange mechanism on cyanopropyl (CN), sulfonic cation exchange
(SCX), mixed phase (MPH) (C8 + SCX), and nonendcapped C18 (C18NE) solid-phase
extraction (SPE) columns. Results indicate that compound A (by contrast with
clenbuterol) is not efficiently retained on the CN, SCX, and MPH SPE columns
(recovery < 10%). This finding thus leads to the development of a gas chromatography–tandem
mass spectrometry procedure based on C18NE SPE that is able to purify both agonists
from bovine livers spiked at 0.5, 1.0, and 2.0 ppb with a mean recovery of 93%
for clenbuterol and 92% for compound A.
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